犬细小病毒CPV-YH毒株的分离鉴定及其基因组变异分析

doi:10.11843/j.issn.0366-6964.2017.09.018

Abstract

Abstract:

To analyze genetic variation of canine parvovirus (CPV), a new virus strain was isolated from the feces of a dog infected by CPV. The virus strain, named as CPV-YH, was identified by red cell agglutination assay, morphology, artificial infection of Beagle dogs and molecular biology. The results showed that the isolate induced specific CPE in A-72 cells, and it could agglutinate porcine red blood cells. The virus were round or hexagonal by the electron microscope, and there was no capsule, with a diameter of about 20 nm. Animal regression test showed that the infected dogs had typical symptoms of CPV disease such as hemorrhagic enteritis, liver edema, pulmonary hyperaemia. The complete genome of CPV-YH was 4 921 nt and it shared the highest nucleotide identity (99%) with CPV-LZ2 isolates isolated from Lanzhou in 2011. The amino acid phylogenetic tree of VP2 showed that CPV-YH isolate, 2013-BJ-P27 (China) and UY306 (Uruguay) isolates were on the same small branch. Compared with the CPV strains on GenBank, the homology of VP2 gene nucleotide and amino acid showed 98%-99.4% and 97.1%-99.5% identities, respectively. In conclusion, one CPV mutant strain was successfully isolated, and these data would provide valuable theoretical basis for the epidemic situation and prevention and control of CPV.

CLC Number:

S852.659.2

SUN Ming, DENG Xiao-yu, LIU Qiao-rong, MA Yong-ying, ZHANG Li, LU Hui-ying, LIU Bo-hua, YANG Xin-yan, CHEN Xi-zhao. Isolation, Identification and Genomic Variation Analysis of Canine Parvovirus CPV-YH Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1724-1729.

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Isolation, Identification and Genomic Variation Analysis of Canine Parvovirus CPV-YH Strain

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